※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||||||
Theoretical PI | 5.74 | ||||||||||||||||||||||||||||||||||||||
Molecular Weight | 42778.41 | ||||||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||||||
Protein Name | GDP-mannose 3,5-epimerase 1; GDP-Man 3,5-epimerase 1; OsGME-1 | ||||||||||||||||||||||||||||||||||||||
Gene Name | GME-1; Os10g0417600; LOC_Os10g28200; OsJ_030296; OSJNBa0061K21.15 | ||||||||||||||||||||||||||||||||||||||
Created Date | 2007-07-10 | ||||||||||||||||||||||||||||||||||||||
Organism | Oryza sativa subsp. japonica(Rice) | ||||||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 39947 | ||||||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Catalyzes a reversible epimerization of GDP-D-mannose that precedes the committed step in the biosynthesis of vitamin C (L-ascorbate), resulting in the hydrolysis of the highly energetic glycosyl-pyrophosphoryl linkage. Able to catalyze 2 distinct epimerization reactions and can release both GDP-L-galactose and GDP-L-gulose from GDP-mannose. Sequence Annotation chain 1 378 GDP-mannose 3,5-epimerase 1 nucleotide phosphate-binding region 36 62 NAD region of interest 145 147 Substrate binding region of interest 217 219 Substrate binding region of interest 242 244 Substrate binding active site 175 Proton acceptor binding site 60 NAD binding site 80 NAD binding site 105 Substrate; via carbonyl oxygen binding site 175 NAD binding site 179 NAD binding site 204 Substrate binding site 226 Substrate binding site 307 Substrate binding site 357 Substrate Keyword Ascorbate biosynthesis,Complete proteome,Isomerase,NAD,Reference proteome Sequence Source UniProt Protein Sequence MGSSEKNGTA YGEYTYAELE REQYWPSEKL RISITGAGGF IGSHIARRLK SEGHYIIASD 60 WKKNEHMTED MFCHEFHLVD LRVMDNCLKV TNGVDHVFNL AADMGGMGFI QSNHSVIMYN 120 NTMISFNMLE AARINGVKRF FYASSACIYP EFKQLETNVS LKESDAWPAE PQDAYGLEKL 180 ATEELCKHYT KDFGIECRVG RFHNIYGPFG TWKGGREKAP AAFCRKAQTS TDRFEMWGDG 240 LQTRSFTFID ECVEGVLRLT KSDFREPVNI GSDEMVSMNE MAEIILSFED RELPIHHIPG 300 PEGVRGRNSD NTLIKEKLGW APTMKLKDGL RFTYFWIKEQ IEKEKTQGVD IAGYGSSKVV 360 STQAPVQLGS LRAADGKE 378 Gene Ontology GO:0005829; C: cytosol; IEA: EnsemblPlants/Gramene GO:0050662; F: coenzyme binding; IEA: InterPro GO:0047918; F: GDP-mannose 3,5-epimerase activity; IEA: UniProtKB-EC GO:0019853; P: L-ascorbic acid biosynthetic process; IEA: UniProtKB-KW Interpro Pfam Pfam; PF01370; Epimerase SMART PROSITE PRINTS |