※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
4.54 
Molecular Weight
33888.85 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
ATPase ASNA1 homolog; Arsenical pump-driving ATPase homolog; Arsenite-stimulated ATPase 
Gene Name
VITISV_018248 
Created Date
2007-06-12 
Organism
Vitis vinifera(Grape) 
NCBI Taxa ID
29760 
Phosphorylation Sites
Position
Peptide
Source
References
3
*****MASDQELSGT
dbPPT, P3DB
[1, 2]
Functional Description
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). 
Sequence Annotation
nucleotide phosphate-binding region 28 35 ATP
active site 57
binding site 226 ATP
binding site 253 ATP
Keyword
ATP-binding,Cytoplasm,Endoplasmic reticulum,Hydrolase,Metal-binding,Nucleotide-binding,Transport,Zinc 
Sequence Source
UniProt 
Protein Sequence
MASDQELSGT VQNILDQESL KWVFVGGKGG VGKTTCSSIL SILLSRVRSS VLIISTDPAH 60
NLSDAFQQRF TKAPTLVNGF SNLYAMEVDP SVENEELPGD GMDNLFSELA NAIPGIDEAM 120
SFAEMLKLVQ TMDYSVIVFD TAPTGHTLRL LQFPSTLEKG LAKMMSLKNK FGGLLNQMTR 180
LFGVDDEFGE DALLGRLEGM KDVIEQVNKQ FKDPDLTTFV CVCIPEFLSL YETERLVQEL 240
NKFEIDSHNV IINQVLYDEE VVESKLLKAR MRMQQKYLDQ FYMLYDDFHI TKLPLLPEEV 300
D 301 
Gene Ontology
GO:0005783; C: endoplasmic reticulum; IEA: UniProtKB-SubCell
GO:0005524; F: ATP binding; IEA: UniProtKB-HAMAP
GO:0016887; F: ATPase activity; IEA: InterPro
GO:0046872; F: metal ion binding; IEA: UniProtKB-KW
GO:0045048; P: protein insertion into ER membrane; IEA: UniProtKB-HAMAP
GO:0006810; P: transport; IEA: UniProtKB-KW 
Interpro
InterPro; IPR025723; Anion-transp_ATPase-like_dom
InterPro; IPR016300; ATPase_ArsA/GET3
InterPro; IPR027542; ATPase_ArsA/GET3_euk
InterPro; IPR027417; P-loop_NTPase
Pfam
Pfam; PF02374; ArsA_ATPase 
SMART
 
PROSITE
 
PRINTS