※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||
Theoretical PI | 7.1 | ||||||||||||||||||||||||||||||
Molecular Weight | 53382.1 | ||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||
Protein Name | Adenylosuccinate synthetase, chloroplastic; AMPSase; AdSS; IMP--aspartate ligase | ||||||||||||||||||||||||||||||
Gene Name | PURA; VIT_08s0007g08130 | ||||||||||||||||||||||||||||||
Created Date | 2010-08-10 | ||||||||||||||||||||||||||||||
Organism | Vitis vinifera(Grape) | ||||||||||||||||||||||||||||||
NCBI Taxa ID | 29760 | ||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity). Sequence Annotation transit peptide 1 37 Chloroplast nucleotide phosphate-binding region 76 82 GTP nucleotide phosphate-binding region 104 106 GTP nucleotide phosphate-binding region 395 397 GTP nucleotide phosphate-binding region 478 480 GTP region of interest 77 80 IMP binding region of interest 102 105 IMP binding region of interest 363 369 Substrate binding active site 77 Proton acceptor active site 105 Proton donor metal ion-binding site 77 Magnesium metal ion-binding site 104 Magnesium; via carbonyl oxygen binding site 194 IMP binding site 208 IMP; shared with dimeric partner binding site 288 IMP binding site 303 IMP binding site 367 IMP binding site 369 GTP Keyword Chloroplast,Complete proteome,GTP-binding,Ligase,Magnesium,Metal-binding,Nucleotide-binding,Plastid,Purine biosynthesis,Reference proteome,Transit peptide Sequence Source UniProt Protein Sequence MNISSLTLDS NPISRTFSAA NPRTQRSPFI RHRNVVVCSV KSVPSSPSLV AAPAGEGVNR 60 IESLSQVSAV LGCQWGDEGK GKLVDILAKH FDIVARCQGG ANAGHTIYNS EGKKFALHLV 120 PSGILNEETM CVIGNGVVVH LPGLFEEIDG LESNGVSCKG RILVSDRAHL LFDFHQVVDG 180 LREAELAKSF IGTTKRGIGP CYSSKVIRNG IRVCDLRHMD TFPQKLDLLL SDAASRFKGF 240 EYGPNMLKEE VEKYKRFTER LEPFITDTVD FMNESISQKK KILVEGGQAT MLDIDFGTYP 300 FVTSSSPSAG GICTGLGIAP RVLGDLVGVV KAYNTRVGSG PFPTENLGRV GDQLRFAGQE 360 FGTTTGRPRR CGWLDIVALK YSCQINGFSS LNLTKLDVLS DLSEIWLGVG YKEIDGKSIK 420 SFPADLRALE QLQVEYEILP GWKTDISGFK NYSDLPKAAQ QYVERIEELV GVPIHYIGVG 480 PGREALIYK 489 Gene Ontology GO:0048046; C: apoplast; IEA: EnsemblPlants/Gramene GO:0009570; C: chloroplast stroma; IEA: EnsemblPlants/Gramene GO:0004019; F: adenylosuccinate synthase activity; IEA: UniProtKB-HAMAP GO:0005525; F: GTP binding; IEA: UniProtKB-HAMAP GO:0000287; F: magnesium ion binding; IEA: UniProtKB-HAMAP GO:0044208; P: 'de novo' AMP biosynthetic process; IEA: UniProtKB-UniPathway GO:0046686; P: response to cadmium ion; IEA: EnsemblPlants/Gramene Interpro Pfam Pfam; PF00709; Adenylsucc_synt SMART SMART; SM00788; Adenylsucc_synt PROSITE PRINTS |