※ dbPPT Protein Information
| Tag | Content | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UniProt Accession | |||||||||||||||||||||||||||||||
Theoretical PI | 7.98 | ||||||||||||||||||||||||||||||
Molecular Weight | 54705.71 | ||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||
Protein Name | Adenylosuccinate synthetase, chloroplastic; AMPSase; AdSS; IMP--aspartate ligase | ||||||||||||||||||||||||||||||
Gene Name | PURA; 35D7.6 | ||||||||||||||||||||||||||||||
Created Date | 2012-09-05 | ||||||||||||||||||||||||||||||
Organism | Triticum aestivum(Wheat) | ||||||||||||||||||||||||||||||
NCBI Taxa ID | 4565 | ||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity). Sequence Annotation transit peptide 1 39 Chloroplast nucleotide phosphate-binding region 75 81 GTP nucleotide phosphate-binding region 103 105 GTP nucleotide phosphate-binding region 409 411 GTP nucleotide phosphate-binding region 492 494 GTP region of interest 76 79 IMP binding region of interest 101 104 IMP binding region of interest 377 383 Substrate binding active site 76 Proton acceptor active site 104 Proton donor metal ion-binding site 76 Magnesium metal ion-binding site 103 Magnesium; via carbonyl oxygen binding site 193 IMP binding site 207 IMP; shared with dimeric partner binding site 287 IMP binding site 302 IMP binding site 381 IMP binding site 383 GTP Keyword Chloroplast,GTP-binding,Ligase,Magnesium,Metal-binding,Nucleotide-binding,Plastid,Purine biosynthesis,Transit peptide Sequence Source UniProt Protein Sequence MPVSAFLSLD PAAYLLPAAR PRPRPPAPRL RLRRVVVLRA ASVSAVAEES SAAAAARGRL 60 ESLSQVAGVL GTQWGDEGKG KLVDILAQRF DVVARCQGGA NAGHTIYNSE GKKFSLHLVP 120 SGILNENTQC VIGNGAVVHL PGFFKEIDGL ESNGVSCKGR ILVSDRAHLL FDFHQVVDGL 180 REVELGNSFI GTTKRGIGPC YSNKVIRNGL RVSDLRHMDT FGAKLNTLLK DAATRFKGFE 240 YSSKTLKEEV EKYERFAERL GPYITDTVHF MNESILQKKK ILVEGGQATM LDIDFGTYPF 300 VTSSSPSAGG ICTGLGIAPR TLGDIIGVPL SLPLQNLTYF SVQVKAYTTR VGSGPFPTEL 360 LGKTGDLLRA SGMEFGTTTG RPRRCGWLDI VALRYCCQIN GFSSLNLTKL DVLTGLKEIK 420 LGTSYCTEDG KAIDSFPADL DLLEQTKVKY EALPGWEEDI SSVRDYDDLP ETARLYVERI 480 EELVGIPVHY IGVGPGRDAL IYK 503 Gene Ontology GO:0009507; C: chloroplast; IEA: UniProtKB-HAMAP GO:0004019; F: adenylosuccinate synthase activity; IEA: UniProtKB-HAMAP GO:0005525; F: GTP binding; IEA: UniProtKB-HAMAP GO:0000287; F: magnesium ion binding; IEA: UniProtKB-HAMAP GO:0044208; P: 'de novo' AMP biosynthetic process; IEA: UniProtKB-UniPathway Interpro Pfam Pfam; PF00709; Adenylsucc_synt SMART SMART; SM00788; Adenylsucc_synt PROSITE PRINTS |