※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
6.6 
Molecular Weight
102453.13 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Nitrate reductase [NADH]; NR 
Gene Name
NIA 
Created Date
1990-08-01 
Organism
Solanum lycopersicum(Tomato Lycopersicon esculentum) 
NCBI Taxa ID
4081 
Phosphorylation Sites
Position
Peptide
Source
References
529
TLKKSISTPFMNTAS
dbPPT
[1]
Functional Description
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria. 
Sequence Annotation
chain 1 911 Nitrate reductase [NADH]
domain 536 611 Cytochrome b5 heme-binding
domain 654 766 FAD-binding FR-type
metal ion-binding site 188 Molybdenum
metal ion-binding site 571 Iron (heme axial ligand)
metal ion-binding site 594 Iron (heme axial ligand)
disulfide bond 427 Interchain
Keyword
Complete proteome,Disulfide bond,FAD,Flavoprotein,Heme,Iron,Metal-binding,Molybdenum,NAD,Nitrate assimilation,Oxidoreductase,Reference proteome 
Sequence Source
UniProt 
Protein Sequence
MAASVENRQY THLEPGLSGV GRTFKPRPDS PVRGCNFPPS SNHELPFQKK QNPPIYLDYS 60
SSEDEDDDDE KNEYVQMIKK GKTELEPSIH DTRDEGTADN WIERNFSLIR LTGKHPFNSE 120
PPLSRLMHHG FITPVPLHYV RNHGPVPKAS WSDWTVEVTG LVKRPMKFTM DQLVNEFPSR 180
EFPVTLVCAG NRRKEQNMVK QTIGFNWGAA AVSTTVWRGV PLRALLKRCG VQSKKKGALN 240
VCFEGSDVLP GGGGSKYGTS IKKEFAMDPS RDIIVAYMQN GEMLSPDHGF PVRMIIPGFI 300
GGRMVKWLKR IVVTTQESES YYHYKDNRVL PPHVDAELAN AEAWWYKPEY IINELNINSV 360
ITTPCHEEIL PINAWTTQRP YTLRGYAYSG GGKKVTRVEV TLDGGETWSV CTLDHPEKPT 420
KYGKYWCWCF WSLEVEVLDL LSAKEIAVRA TDETLNTQPE KLIWNVMGMM NNCWFRVKMN 480
VCKPHKGEIG IVFEHPTQPG NQSGGWMAKE RHLEISAVAP PTLKKSISTP FMNTASKMYS 540
MSEVRKHNSS DSAWIIVHGH IYDASRFLKD HPGGVDSILI NAGTDCTEEF DAIHSDKAKK 600
LLEDFRIGEL ITTGYTSDSS PNSSVHGSSS ISSFLAPIKE LVQTPTRSVA LIPREKIPCK 660
LVDKQSISHD VRKFKFALPS EDQVLGLPVG KHIFLCATVD DKLCMRAYTP TSTVDEVGFF 720
ELVVKIYFKG VHPKFPNGGQ MSQHLDSLPI GAFLDVKGPL GHIEYQGKGN FLVHGKQKFA 780
KKLAMIAGGT GITPVYQVMQ SILKDPEDDT EMYVVYANRT EDDILLKDEL DAWAEQVPNR 840
VKVWYVVQES ITQGWKYSTG FVTESILREH IPEPSHTTLA LACGPPPMIQ FAINPNLEKM 900
GYDIKEELLV F 911 
Gene Ontology
GO:0005829; C: cytosol; IEA: EnsemblPlants/Gramene
GO:0005886; C: plasma membrane; IEA: EnsemblPlants/Gramene
GO:0005773; C: vacuole; IEA: EnsemblPlants/Gramene
GO:0009055; F: electron carrier activity; IEA: InterPro
GO:0050660; F: flavin adenine dinucleotide binding; IEA: InterPro
GO:0020037; F: heme binding; IEA: InterPro
GO:0005506; F: iron ion binding; IEA: InterPro
GO:0030151; F: molybdenum ion binding; IEA: InterPro
GO:0043546; F: molybdopterin cofactor binding; IEA: InterPro
GO:0009703; F: nitrate reductase (NADH) activity; IEA: UniProtKB-EC
GO:0042128; P: nitrate assimilation; IEA: UniProtKB-KW
GO:0006809; P: nitric oxide biosynthetic process; IEA: EnsemblPlants/Gramene
GO:0009416; P: response to light stimulus; IEA: EnsemblPlants/Gramene
GO:0009610; P: response to symbiotic fungus; IEA: EnsemblPlants/Gramene 
Interpro
InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd
InterPro; IPR018506; Cyt_B5_heme-BS
InterPro; IPR017927; Fd_Rdtase_FAD-bd
InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase
InterPro; IPR014756; Ig_E-set
InterPro; IPR005066; MoCF_OxRdtse_dimer
InterPro; IPR008335; Mopterin_OxRdtase_euk
InterPro; IPR001834; NADH-Cyt_B5_reductase
InterPro; IPR012137; Nitr_rd_NADH
InterPro; IPR008333; OxRdtase_FAD-bd_dom
InterPro; IPR001433; OxRdtase_FAD/NAD-bd
InterPro; IPR000572; OxRdtase_Mopterin-bd_dom
InterPro; IPR022407; OxRdtase_Mopterin_BS
InterPro; IPR017938; Riboflavin_synthase-like_b-brl
Pfam
Pfam; PF00173; Cyt-b5
Pfam; PF00970; FAD_binding_6
Pfam; PF03404; Mo-co_dimer
Pfam; PF00175; NAD_binding_1
Pfam; PF00174; Oxidored_molyb 
SMART
 
PROSITE
PS00191; CYTOCHROME_B5_1
PS50255; CYTOCHROME_B5_2
PS51384; FAD_FR
PS00559; MOLYBDOPTERIN_EUK 
PRINTS
PR00406; CYTB5RDTASE
PR00363; CYTOCHROMEB5
PR00407; EUMOPTERIN
PR00371; FPNCR