※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
6.04 
Molecular Weight
101770.47 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Nitrate reductase [NADH]; NR 
Gene Name
 
Created Date
1992-08-01 
Organism
Hordeum vulgare(Barley) 
NCBI Taxa ID
4513 
Phosphorylation Sites
Position
Peptide
Source
References
37
GIRRRADSPVRGCGF
dbPPT
[1]
529
PGLKRSTSTPFMNTA
dbPPT
[1]
530
GLKRSTSTPFMNTAG
dbPPT
[1]
652
GAPIALSSPREKVPC
dbPPT
[1]
Functional Description
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria. 
Sequence Annotation
chain 1 915 Nitrate reductase [NADH]
domain 538 613 Cytochrome b5 heme-binding
domain 654 767 FAD-binding FR-type
metal ion-binding site 189 Molybdenum
metal ion-binding site 573 Iron (heme axial ligand)
metal ion-binding site 596 Iron (heme axial ligand)
disulfide bond 428 Interchain
Keyword
Disulfide bond,FAD,Flavoprotein,Heme,Iron,Metal-binding,Molybdenum,NAD,Nitrate assimilation,Oxidoreductase 
Sequence Source
UniProt 
Protein Sequence
MAASVEPRQP FGRLDAPATA PTARAPGSNG IRRRADSPVR GCGFPSLISP PRKGPVAEEE 60
EDDDDEDDEG HEDWREAYGS HLQLEVEPST RDPRDEGTAD AWIERNPSLI RLTGKHPLNC 120
EPPLARLMHH GFITPAPLHY VRNHGAVPRG DWATWTVEVT GLVRRPARLT MDELANGFPA 180
AEVPATLVCA GNRRKEQNMV QQTVGFNWGA AGVSTSVWRG ARLRDVLLRC GVMSKKGQAL 240
NVCFEGAEDL PGGGGSKYGT SVSREWAMDP SRDIILPYAQ NGEPLLPDHG YPVRVLIPGC 300
IGGRMVKWVR RIVVTTAESD NYYHFKDNRV LPSHVDAELA NAEAWWYRPE YIINELNTNC 360
VITTPGHDEI LPINAFTTQR AYTIKGYAYS GGGKKITRVE VTLDGGESWM LCTLDIPEKP 420
NKYGRYWCWC FWSVEIEVLD LLGAKEVAVR TWDQTHNTQP EKLIWNLMGM MNNCWFKIKV 480
NVCRPHKGEI GLVFEHPTQP GNQTGGWMAR QKHLETAEAA APGLKRSTST PFMNTAGDKQ 540
FTMSEVRKHG SKESAWIVVH GHVYDCTAFL KDHPGGADSI LINAGSDCTE EFDAIHSDKA 600
KALLDTYRIG ELITTGTGYN SDNSVHGGSS LSHLAPIREA TKVAGAPIAL SSPREKVPCR 660
LVDKKELSHD VRLFRFALPS SDQVLGLPVG KHIFVCATID GKLCMRAYTP TSMVDEIGQF 720
ELLVKVYFRD EHPKFPNGGL MTQYLESLQV GSSYIDVKGP LGHVEYTGRG NFVINGKQRR 780
ARRLAMICGG SGITPMYQVI QAVLRDQPED ETEMHLVYAN RSEDDILLRD ELDRWAAEYP 840
DRLKVWYVID QVKRPEDGWK FSVGFVTEDI LRAHVPEGGD DTLALACGPP PMIKFAISPN 900
LEKMKYDMAN SFISF 915 
Gene Ontology
GO:0005829; C: cytosol; IEA: EnsemblPlants/Gramene
GO:0005886; C: plasma membrane; IEA: EnsemblPlants/Gramene
GO:0005773; C: vacuole; IEA: EnsemblPlants/Gramene
GO:0009055; F: electron carrier activity; IEA: InterPro
GO:0050660; F: flavin adenine dinucleotide binding; IEA: InterPro
GO:0020037; F: heme binding; IEA: InterPro
GO:0005506; F: iron ion binding; IEA: InterPro
GO:0030151; F: molybdenum ion binding; IEA: InterPro
GO:0043546; F: molybdopterin cofactor binding; IEA: InterPro
GO:0009703; F: nitrate reductase (NADH) activity; IEA: UniProtKB-EC
GO:0042128; P: nitrate assimilation; IEA: UniProtKB-KW
GO:0006809; P: nitric oxide biosynthetic process; IEA: EnsemblPlants/Gramene
GO:0009416; P: response to light stimulus; IEA: EnsemblPlants/Gramene
GO:0009610; P: response to symbiotic fungus; IEA: EnsemblPlants/Gramene 
Interpro
InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd
InterPro; IPR018506; Cyt_B5_heme-BS
InterPro; IPR017927; Fd_Rdtase_FAD-bd
InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase
InterPro; IPR014756; Ig_E-set
InterPro; IPR005066; MoCF_OxRdtse_dimer
InterPro; IPR008335; Mopterin_OxRdtase_euk
InterPro; IPR001834; NADH-Cyt_B5_reductase
InterPro; IPR012137; Nitr_rd_NADH
InterPro; IPR008333; OxRdtase_FAD-bd_dom
InterPro; IPR001433; OxRdtase_FAD/NAD-bd
InterPro; IPR000572; OxRdtase_Mopterin-bd_dom
InterPro; IPR022407; OxRdtase_Mopterin_BS
InterPro; IPR017938; Riboflavin_synthase-like_b-brl
Pfam
Pfam; PF00173; Cyt-b5
Pfam; PF00970; FAD_binding_6
Pfam; PF03404; Mo-co_dimer
Pfam; PF00175; NAD_binding_1
Pfam; PF00174; Oxidored_molyb 
SMART
 
PROSITE
PS00191; CYTOCHROME_B5_1
PS50255; CYTOCHROME_B5_2
PS51384; FAD_FR
PS00559; MOLYBDOPTERIN_EUK 
PRINTS
PR00406; CYTB5RDTASE
PR00363; CYTOCHROMEB5
PR00407; EUMOPTERIN
PR00371; FPNCR