※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||
Theoretical PI | 5.25 | ||||||||||||||||||||||||||||||||||
Molecular Weight | 79532.58 | ||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||
Protein Name | Pyrophosphate-energized vacuolar membrane proton pump; Pyrophosphate-energized inorganic pyrophosphatase; H(+)-PPase | ||||||||||||||||||||||||||||||||||
Gene Name | |||||||||||||||||||||||||||||||||||
Created Date | 1995-02-01 | ||||||||||||||||||||||||||||||||||
Organism | Hordeum vulgare(Barley) | ||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 4513 | ||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Contributes to the transtonoplast (from cytosol to vacuole lumen) H(+)-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H(+)-ATPase on the same membrane. Sequence Annotation chain 1 762 Pyrophosphate-energized vacuolar membrane proton pump topological domain 1 6 Intravacuolar transmembrane region 7 33 Helical; topological domain 34 81 Cytoplasmic transmembrane region 82 111 Helical; topological domain 112 131 Intravacuolar transmembrane region 132 159 Helical; topological domain 160 182 Cytoplasmic transmembrane region 183 212 Helical; topological domain 213 215 Intravacuolar transmembrane region 216 244 Helical; topological domain 245 282 Cytoplasmic transmembrane region 283 308 Helical; topological domain 309 316 Intravacuolar transmembrane region 317 342 Helical; topological domain 343 350 Cytoplasmic transmembrane region 351 378 Helical; topological domain 379 397 Intravacuolar transmembrane region 398 421 Helical; topological domain 422 443 Cytoplasmic transmembrane region 444 468 Helical; topological domain 469 474 Intravacuolar transmembrane region 475 501 Helical; topological domain 502 530 Cytoplasmic transmembrane region 531 559 Helical; topological domain 560 569 Intravacuolar transmembrane region 570 598 Helical; topological domain 599 627 Cytoplasmic transmembrane region 628 656 Helical; topological domain 657 Intravacuolar transmembrane region 658 685 Helical; topological domain 686 728 Cytoplasmic transmembrane region 729 754 Helical; topological domain 755 762 Intravacuolar metal ion-binding site 249 Magnesium 1 metal ion-binding site 249 Magnesium 2 metal ion-binding site 253 Magnesium 1 metal ion-binding site 279 Magnesium 3 metal ion-binding site 503 Magnesium 3 metal ion-binding site 530 Magnesium 4 metal ion-binding site 687 Magnesium 4 metal ion-binding site 723 Magnesium 2 binding site 246 Substrate binding site 726 Substrate site 238 Important for proton transport site 283 Important for proton transport site 290 Important for proton transport site 297 Important for proton transport site 727 Important for proton transport site 738 Important for proton transport disulfide bond 121 128 Keyword Disulfide bond,Hydrogen ion transport,Hydrolase,Ion transport,Magnesium,Membrane,Metal-binding,Transmembrane,Transmembrane helix,Transport,Vacuole Sequence Source UniProt Protein Sequence MAILGELGTE ILIPVCGVIG IVFAVAQWFI VSKVKVTPGA ASAAAGAKNG YGDYLIEEEE 60 GLNDHNVVVK CAEIQTAISE GATSFLFTMY QYVGMFMVVF AAIIFLFLGS IEGFSTKGQP 120 CTYSKGTCKP ALYTALFSTA SFLLGAITSL VSGFLGMKIA TYANARTTLE ARKGVGKAFI 180 TAFRSGAVMG FLLSSSGLVV LYITINVFKM YYGDDWEGLF ESITGYGLGG SSMALFGRVG 240 GGIYTKAADV GADLVGKVER NIPEDDPRNP AVIADNVGDN VGDIAGMGSD LFGSYAESSC 300 AALVVASISS FGINHDFTAM CYPLLVSSVG IIVCLLTTLF ATDFFEIKAA NEIEPALKKQ 360 LIISTALMTV GVAVISWLAL PAKFTIFNFG AQKEVSNWGL FFCVAVGLWA GLIIGFVTEY 420 YTSNAYSPVQ DVADSCRTGA ATNVIFGLAL GYKSVIIPIF AIAVSIYVSF SIAAMYGIAM 480 AALGMLSTMA TGLAIDAYGP ISDNAGGIAE MAGMSHRIRE RTDALDAAGN TTAAIGKGFA 540 IGSAALVSLA LFGAFVSRAG VKVVDVLSPK VFIGLIVGAM LPYWFSAMTM KSVGSAALKM 600 VEEVRRQFNT IPGLMEGTAK PDYATCVKIS TDASIKEMIP PGALVMLTPL IVGTLFGVET 660 LSGVLAGALV SGVQIAISAS NTGGAWDNAK KYIEAGNSEH ARSLGPKGSD CHKAAVIGDT 720 IGDPLKDTSG PSLNILIKLM AVESLVFAPF FATYGGLLFK YI 762 Gene Ontology GO:0016021; C: integral component of membrane; IEA: UniProtKB-KW GO:0005774; C: vacuolar membrane; IEA: UniProtKB-SubCell GO:0009678; F: hydrogen-translocating pyrophosphatase activity; IEA: InterPro GO:0004427; F: inorganic diphosphatase activity; IEA: UniProtKB-EC GO:0046872; F: metal ion binding; IEA: UniProtKB-KW GO:0015992; P: proton transport; IEA: UniProtKB-KW Interpro InterPro; IPR004131; PPase-energised_H-pump Pfam Pfam; PF03030; H_PPase SMART PROSITE PRINTS |