UniProt Accession

Q0J8A4; B7EIW3; Q42977; Q6ZK60; G3PC1_ORYSJ 

Theoretical PI

6.61 

Molecular Weight

36412.65 

Genbank Protein ID

AK071097;AK103777;EF122472;AP008214;CM000145;GQ848031;AP003886 

Genbank Nucleotide ID

ADM86844.1;EAZ41375.1;BAF22811.1;BAD08850.1;BAG96258.1;ABL74559.1;BAG92310.1 

Protein Name

Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic; PP38 

Gene Name

GAPC1; GAPC; GAPDH; GPC; Os08g0126300; LOC_Os08g03290; OJ1163_G08.15; OsJ_024858 

Created Date

2007-07-10 

Organism

Oryza sativa subsp. japonica(Rice) 

NCBI Taxa ID

39947 

Phosphorylation Sites

Position	Peptide	Source	References
212	SFNIIPSSTGAAKAV	dbPPT, P3DB	[1, 2]
205	WRGGRAASFNIIPSS	dbPPT, P3DB	[1, 2, 3]

Functional Description

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism (By similarity). 

Sequence Annotation

initiator methionine 1 Removed
chain 2 337 Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic
nucleotide phosphate-binding region 13 14 NAD
region of interest 153 155 Glyceraldehyde 3-phosphate binding
region of interest 213 214 Glyceraldehyde 3-phosphate binding
active site 154 Nucleophile
binding site 35 NAD
binding site 82 NAD; via carbonyl oxygen
binding site 184 Glyceraldehyde 3-phosphate
binding site 236 Glyceraldehyde 3-phosphate
binding site 318 NAD
site 181 Activates thiol group during catalysis
strand 4 9
helix 13 23
strand 26 34
strand 36 38
helix 40 48
turn 51 53
strand 61 65
strand 68 71
strand 74 79
helix 84 86
helix 89 92
strand 96 99
strand 101 103
helix 107 110
helix 112 115
strand 120 125
strand 128 130
turn 135 137
helix 139 141
strand 148 150
helix 154 170
strand 172 181
strand 187 191
helix 198 201
helix 204 206
strand 209 212
helix 215 222
helix 224 226
strand 229 236
strand 243 253
helix 257 269
turn 270 272
strand 276 279
helix 285 288
strand 294 298
turn 299 301
strand 303 306
strand 309 316
helix 320 335

Keyword

3D-structure,Complete proteome,Cytoplasm,Direct protein sequencing,Glycolysis,NAD,Oxidoreductase,Reference proteome 

Sequence Source

UniProt 

Protein Sequence

Gene Ontology

GO:0048046; C: apoplast; IEA: EnsemblPlants/Gramene
GO:0009507; C: chloroplast; IEA: EnsemblPlants/Gramene
GO:0005740; C: mitochondrial envelope; IEA: EnsemblPlants/Gramene
GO:0005886; C: plasma membrane; IEA: EnsemblPlants/Gramene
GO:0005774; C: vacuolar membrane; IEA: EnsemblPlants/Gramene
GO:0005507; F: copper ion binding; IEA: EnsemblPlants/Gramene
GO:0004365; F: glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA: UniProtKB-EC
GO:0008886; F: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity; IEA: EnsemblPlants/Gramene
GO:0051287; F: NAD binding; IEA: InterPro
GO:0050661; F: NADP binding; IEA: InterPro
GO:0006006; P: glucose metabolic process; IEA: InterPro
GO:0006096; P: glycolytic process; IEA: UniProtKB-UniPathway
GO:0046686; P: response to cadmium ion; IEA: EnsemblPlants/Gramene
GO:0009408; P: response to heat; IEA: EnsemblPlants/Gramene
GO:0042542; P: response to hydrogen peroxide; IEA: EnsemblPlants/Gramene
GO:0051775; P: response to redox state; IEA: EnsemblPlants/Gramene
GO:0009651; P: response to salt stress; IEA: EnsemblPlants/Gramene
GO:0009744; P: response to sucrose; IEA: EnsemblPlants/Gramene
GO:0048316; P: seed development; IEA: EnsemblPlants/Gramene 

Interpro

InterPro; IPR020831; GlycerAld/Erythrose_P_DH
InterPro; IPR020830; GlycerAld_3-P_DH_AS
InterPro; IPR020829; GlycerAld_3-P_DH_cat
InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd
InterPro; IPR006424; Glyceraldehyde-3-P_DH_1
InterPro; IPR016040; NAD(P)-bd_dom

Pfam

Pfam; PF02800; Gp_dh_C
Pfam; PF00044; Gp_dh_N 

SMART

SMART; SM00846; Gp_dh_N 

PROSITE

PS00071; GAPDH 

PRINTS

PR00078; G3PDHDRGNASE