※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||
Theoretical PI | 6.39 | ||||||||||||||||||||||||||||||
Molecular Weight | 52135.32 | ||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||
Protein Name | Adenylosuccinate synthetase 1, chloroplastic; AMPSase 1; AdSS 1; IMP--aspartate ligase 1 | ||||||||||||||||||||||||||||||
Gene Name | PURA1; Os03g0174500; LOC_Os03g07840; OsJ_09613 | ||||||||||||||||||||||||||||||
Created Date | 2010-10-05 | ||||||||||||||||||||||||||||||
Organism | Oryza sativa subsp. japonica(Rice) | ||||||||||||||||||||||||||||||
NCBI Taxa ID | 39947 | ||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity). Sequence Annotation transit peptide 1 47 Chloroplast chain 48 488 Adenylosuccinate synthetase 1, chloroplastic nucleotide phosphate-binding region 75 81 GTP nucleotide phosphate-binding region 103 105 GTP nucleotide phosphate-binding region 394 396 GTP nucleotide phosphate-binding region 477 479 GTP region of interest 76 79 IMP binding region of interest 101 104 IMP binding region of interest 362 368 Substrate binding active site 76 Proton acceptor active site 104 Proton donor metal ion-binding site 76 Magnesium metal ion-binding site 103 Magnesium; via carbonyl oxygen binding site 193 IMP binding site 207 IMP; shared with dimeric partner binding site 287 IMP binding site 302 IMP binding site 366 IMP binding site 368 GTP Keyword Chloroplast,Complete proteome,GTP-binding,Ligase,Magnesium,Metal-binding,Nucleotide-binding,Plastid,Purine biosynthesis,Reference proteome,Transit peptide Sequence Source UniProt Protein Sequence MSLSTVNHAA AAAAAAAGPG KSFSAAAPAA PSVRLPRTRA PAAAAVSAAA VGADRAADRV 60 SALSQVSGVL GSQWGDEGKG KLVDVLAPRF DIVARCQGGA NAGHTIYNSE GKKFALHLVP 120 SGILHEGTLC VVGNGAVIHV PGFFNEIDGL ESNGVNCNGR ILVSDRAHLL FDLHQAVDGL 180 REAELANSFI GTTKRGIGPC YSSKVTRNGL RVCDLRHMDT FGDKLDVLFK DATSRFEGFE 240 YSKSMLREEV ERYKRFAERL EPFIADTVHL LNESIQQKKK ILVEGGQATM LDIDFGTYPF 300 VTSSSPSAGG ICTGLGIAPR CIGDLIGVVK AYTTRVGSGP FPTELFGEEG DLLRKSGMEF 360 GTTTGRPRRC GWLDIVALKY CCEINGFSSL NLTKLDVLSG LPEVKLGVSY NQPDGQKLQS 420 FPGDLDTLEQ VQVKYEVLPG WQSDISSVRS YSELPLAAQR YVERIEELVG VPVHYIGVGP 480 GRDALIYK 488 Gene Ontology GO:0048046; C: apoplast; IEA: EnsemblPlants/Gramene GO:0009570; C: chloroplast stroma; IEA: EnsemblPlants/Gramene GO:0004019; F: adenylosuccinate synthase activity; IEA: UniProtKB-HAMAP GO:0005525; F: GTP binding; IEA: UniProtKB-HAMAP GO:0000287; F: magnesium ion binding; IEA: UniProtKB-HAMAP GO:0044208; P: 'de novo' AMP biosynthetic process; IEA: UniProtKB-UniPathway GO:0046686; P: response to cadmium ion; IEA: EnsemblPlants/Gramene Interpro Pfam Pfam; PF00709; Adenylsucc_synt SMART SMART; SM00788; Adenylsucc_synt PROSITE PRINTS |