※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||
Theoretical PI | 5.57 | ||||||||||||||||||||||||||||||||||
Molecular Weight | 40753.52 | ||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||
Protein Name | Isoflavone 4'-O-methyltransferase; MtHI4'OMT; 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase | ||||||||||||||||||||||||||||||||||
Gene Name | HI4'OMT | ||||||||||||||||||||||||||||||||||
Created Date | 2011-07-27 | ||||||||||||||||||||||||||||||||||
Organism | Medicago truncatula(Barrel medic Medicago tribuloides) | ||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 3880 | ||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description 2-hydroxyisoflavanone 4'-O-methyltransferase involved in the biosynthesis of the phytoalexin medicarpin. Has also an in vitro (+)-6a-hydroxymaackiain-3-0-methyltransferase activity, converting the pterocarpan 6a-hydroxymaackiain into pisatin. No activity with di- or trihydroxylated isoflavones, including daidzein and genistein, or with (-)-medicarpin and maackiain. The dual activity for either 3- or 4'-O-methylation depends upon substrate availability. Sequence Annotation chain 1 364 Isoflavone 4'-O-methyltransferase region of interest 206 209 S-adenosyl-L-methionine binding region of interest 230 231 S-adenosyl-L-methionine binding region of interest 250 251 S-adenosyl-L-methionine binding compositionally biased region 96 99 Poly-Glu active site 268 Proton acceptor binding site 230 S-adenosyl-L-methionine binding site 264 S-adenosyl-L-methionine turn 8 10 helix 16 24 helix 27 39 helix 41 48 helix 54 60 turn 65 67 helix 68 80 strand 83 89 strand 92 95 strand 99 104 helix 106 109 strand 113 115 helix 120 126 helix 129 132 helix 133 137 helix 138 143 helix 150 155 helix 159 163 helix 166 168 helix 169 184 helix 186 192 helix 194 198 strand 201 206 helix 212 220 strand 224 230 helix 232 235 strand 242 248 turn 251 253 strand 259 265 helix 267 269 helix 272 285 helix 286 292 strand 294 299 helix 309 326 helix 333 342 strand 347 353 turn 354 356 strand 357 363 Keyword 3D-structure,Methyltransferase,S-adenosyl-L-methionine,Transferase Sequence Source UniProt Protein Sequence MAFSTNGSEE SELYHAQIHL YKHVYNFVSS MALKSAMELG IADAIHNHGK PMTLSELASS 60 LKLHPSKVNI LHRFLRLLTH NGFFAKTIVK GKEGDEEEEI AYSLTPPSKL LISGKPTCLS 120 SIVKGALHPS SLDMWSSSKK WFNEDKEQTL FECATGESFW DFLNKDSESS TLSMFQDAMA 180 SDSRMFKLVL QENKRVFEGL ESLVDVGGGT GGVTKLIHEI FPHLKCTVFD QPQVVGNLTG 240 NENLNFVGGD MFKSIPSADA VLLKWVLHDW NDEQSLKILK NSKEAISHKG KDGKVIIIDI 300 SIDETSDDRG LTELQLDYDL VMLTMFLGKE RTKQEWEKLI YDAGFSSYKI TPISGFKSLI 360 EVYP 364 Gene Ontology GO:0030746; F: isoflavone 4'-O-methyltransferase activity; IEA: UniProtKB-EC GO:0008171; F: O-methyltransferase activity; IEA: InterPro Interpro Pfam SMART PROSITE PS51683; SAM_OMT_II PRINTS |