※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||
Theoretical PI | 5.74 | ||||||||||||||||||||||||||||||
Molecular Weight | 42132.01 | ||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||
Protein Name | GDP-mannose 3,5-epimerase 2; GDP-Man 3,5-epimerase 2 | ||||||||||||||||||||||||||||||
Gene Name | GME-2; Os11g0591100; LOC_Os11g37890 | ||||||||||||||||||||||||||||||
Created Date | 2006-05-02 | ||||||||||||||||||||||||||||||
Organism | Oryza sativa subsp. japonica(Rice) | ||||||||||||||||||||||||||||||
NCBI Taxa ID | 39947 | ||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Catalyzes a reversible epimerization of GDP-D-mannose that precedes the committed step in the biosynthesis of vitamin C (L-ascorbate), resulting in the hydrolysis of the highly energetic glycosyl-pyrophosphoryl linkage. Able to catalyze 2 distinct epimerization reactions and can release both GDP-L-galactose and GDP-L-gulose from GDP-mannose (By similarity). Sequence Annotation chain 1 371 GDP-mannose 3,5-epimerase 2 nucleotide phosphate-binding region 29 55 NAD region of interest 138 140 Substrate binding region of interest 210 212 Substrate binding region of interest 235 237 Substrate binding active site 168 Proton acceptor binding site 53 NAD binding site 73 NAD binding site 98 Substrate; via carbonyl oxygen binding site 168 NAD binding site 172 NAD binding site 197 Substrate binding site 219 Substrate binding site 300 Substrate binding site 350 Substrate Keyword Ascorbate biosynthesis,Complete proteome,Isomerase,NAD,Reference proteome Sequence Source UniProt Protein Sequence MALNEEYTYV ELEKEPYWPF EKLRISITGA GGFIASHIAR RLKSEGHYII ASDWKKNEHM 60 TEDMFCHEFH LVDLRVMDNC LKVTTGVDHV FNLAADMGGM GFIQSNHSVI MYNNTMISFN 120 MLEAARINGV KRFFYASSAC IYPEFKQLDT VVSLKESDAW PAEPQDAYGL EKLATEELCK 180 HYTKDFGIEC RVGRFHNIYG PFGTWKGGRE KAPAAFCRKA LTSTDRFEMW GDGLQTRSFT 240 FIDECVEGVL RLTKSDFREP VNIGSDEMVS MNEMAEIVLS FENKQLPIHH IPGPEGVRGR 300 NSDNTLIKEK LGWAPTMRLK DGLRITYFWI KEQLEKEKAE GVDLSAYGSS KVVQTQAPVQ 360 LGSLRAADGK E 371 Gene Ontology GO:0050662; F: coenzyme binding; IEA: InterPro GO:0047918; F: GDP-mannose 3,5-epimerase activity; IEA: UniProtKB-EC GO:0019853; P: L-ascorbic acid biosynthetic process; IEA: UniProtKB-KW Interpro Pfam Pfam; PF01370; Epimerase SMART PROSITE PRINTS |