※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
5.45 
Molecular Weight
85431.68 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Two pore calcium channel protein 1; Voltage-dependent calcium channel protein TPC1; HvTPC1 
Gene Name
TPC1 
Created Date
2008-07-01 
Organism
Hordeum vulgare(Barley) 
NCBI Taxa ID
4513 
Phosphorylation Sites
Position
Peptide
Source
References
703
GEEVDIQSPTSGGIK
dbPPT
[1]
23
RGLASSGSRRLSDGG
dbPPT
[1]
27
SSGSRRLSDGGGGQG
dbPPT
[1]
35
DGGGGQGSRKYRRRS
dbPPT
[1]
21
AERGLASSGSRRLSD
dbPPT
[1]
Functional Description
Functions as a voltage-gated inward-rectifying Ca(2+) channel (VDCC) across the plasma membrane that mediates sucrose-induced Ca(2+) influx in autotrophically grown leaf cells. Acts as the major ROS-responsive Ca(2+) channel and is the possible target of Al-dependent inhibition. Plays a regulatory role in defense responses (By similarity). 
Sequence Annotation
chain 1 742 Two pore calcium channel protein 1
topological domain 1 82 Cytoplasmic
transmembrane region 83 103 Helical; Name=S1 of repeat I;
topological domain 104 140 Extracellular
transmembrane region 141 161 Helical; Name=S2 of repeat I;
topological domain 162 176 Cytoplasmic
transmembrane region 177 197 Helical; Name=S3 of repeat I;
topological domain 198 204 Extracellular
transmembrane region 205 226 Helical; Voltage-sensor; Name=S4 of repeat I;
transmembrane region 227 247 Helical; Name=S5 of repeat I;
topological domain 248 258 Extracellular
intramembrane region 259 273 Pore-forming; Name=Pore-forming 1;
topological domain 274 296 Extracellular
transmembrane region 297 317 Helical; Name=S6 of repeat I;
topological domain 318 446 Cytoplasmic
transmembrane region 447 467 Helical; Name=S1 of repeat II;
topological domain 468 480 Extracellular
transmembrane region 481 501 Helical; Name=S2 of repeat II;
topological domain 502 510 Cytoplasmic
transmembrane region 511 531 Helical; Name=S3 of repeat II;
topological domain 532 540 Extracellular
transmembrane region 541 558 Helical; Voltage-sensor; Name=S4 of repeat II;
topological domain 559 582 Cytoplasmic
transmembrane region 583 603 Helical; Name=S5 of repeat II;
topological domain 604 627 Extracellular
intramembrane region 628 642 Pore-forming; Name=Pore-forming 2;
topological domain 643 663 Extracellular
transmembrane region 664 684 Helical; Name=S6 of repeat II;
topological domain 685 742 Cytoplasmic
domain 335 370 EF-hand 1
domain 376 411 EF-hand 2
compositionally biased region 29 34 Poly-Gly
compositionally biased region 673 678 Poly-Leu
glycosylation site 469 N-linked (GlcNAc...)
Keyword
Calcium,Calcium channel,Calcium transport,Glycoprotein,Ion channel,Ion transport,Membrane,Plant defense,Repeat,Transmembrane,Transmembrane helix,Transport,Voltage-gated channel 
Sequence Source
UniProt 
Protein Sequence
MSEAQAPLIT EEAAERGLAS SGSRRLSDGG GGQGSRKYRR RSDALAHGDR YQKAAALVDL 60
AEDGVGIPED VLNDTRFGRA MSFYFVYLRL DWLWSLNIFA LILLNFLEKP LWCRKDALHA 120
CDQRDMYFLG QLPYFSKTES LIYEGLTLVI LVMEILCPLS YEGLNIFWRS TTNKLKILLL 180
FILACDILVF AFSSQPFRLA PYIRVVFLIM TIRELRMCAI TLAGLIGTYL NVLALSLLFL 240
LFASWLAYVT FEDTPQGKTI FSSYGVTLYQ MFVLFTTSNN PDVWVPAYKI SRWYSLFFIV 300
YVLLGVYFLT NLILAVIYDS FKEQFAKQLV QVDAIRKNIL QKAFELIDTN TRGYLDREQC 360
ISLLNELNKY RSLPKTSRED FELIFAELDR SGDFKVTSEE FADLCNTIAI KFQKEPPPSY 420
LEKFPFYHSP VCGRLKSFVR SRTFEYIIVF VLLINLVAVI IETTLDIENS SSQETWQEVE 480
FFLGWIYVAE MALKIFSLGF GAYWMEGQNK FDFVLTWTIF IGETLTFAFP SKLPFLSNGE 540
WIRYLLLGRV LRLTRILLQV QRFRVFVATF FTLMSSLMPY LGIVFCILCM YCSLGLQIFG 600
GIVYAGNPTL EETDLFSNDY LLFNFNDYPS GMVTLFNLLV MGNWQVWMES YWQLTGSSWS 660
LIYFVSFYLI SILLLLNLIV AFVLEAFFAE MELEKGEEVD IQSPTSGGIK KRRSMRVRSK 720
GTMVDILLHH MLSNELDGSQ NS 742 
Gene Ontology
GO:0005794; C: Golgi apparatus; IEA: EnsemblPlants/Gramene
GO:0016021; C: integral component of membrane; IEA: UniProtKB-KW
GO:0000325; C: plant-type vacuole; IEA: EnsemblPlants/Gramene
GO:0005509; F: calcium ion binding; IEA: InterPro
GO:0005245; F: voltage-gated calcium channel activity; IEA: EnsemblPlants/Gramene
GO:0019722; P: calcium-mediated signaling; IEA: EnsemblPlants/Gramene
GO:0006952; P: defense response; IEA: UniProtKB-KW
GO:0080141; P: regulation of jasmonic acid biosynthetic process; IEA: EnsemblPlants/Gramene
GO:0010119; P: regulation of stomatal movement; IEA: EnsemblPlants/Gramene
GO:0009845; P: seed germination; IEA: EnsemblPlants/Gramene 
Interpro
InterPro; IPR027359; Channel_four-helix_dom
InterPro; IPR011992; EF-hand-dom_pair
InterPro; IPR002048; EF_hand_dom
InterPro; IPR005821; Ion_trans_dom
InterPro; IPR028801; TPC1
Pfam
Pfam; PF13499; EF-hand_7
Pfam; PF00520; Ion_trans 
SMART
SMART; SM00054; EFh 
PROSITE
PS50222; EF_HAND_2 
PRINTS