※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||
Theoretical PI | 6.55 | ||||||||||||||||||||||||||||||
Molecular Weight | 38088.1 | ||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||
Protein Name | 2-alkenal reductase (NADP(+)-dependent); Alkenal double bound reductase; Allylic alcohol dehydrogenase 1; allyl-ADH1; Flavin-free double bond reductase; NtDBR; Pulegone reductase; NtRed-1 | ||||||||||||||||||||||||||||||
Gene Name | DBR | ||||||||||||||||||||||||||||||
Created Date | 2013-07-24 | ||||||||||||||||||||||||||||||
Organism | Nicotiana tabacum(Common tobacco) | ||||||||||||||||||||||||||||||
NCBI Taxa ID | 4097 | ||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones. Sequence Annotation chain 1 343 2-alkenal reductase (NADP(+)-dependent) nucleotide phosphate-binding region 165 166 NADP nucleotide phosphate-binding region 282 284 NADP binding site 55 Substrate binding site 80 Substrate binding site 186 NADP; via amide nitrogen binding site 190 NADP binding site 206 NADP binding site 230 NADP binding site 252 NADP binding site 258 NADP binding site 332 NADP strand 4 12 strand 17 19 helix 22 24 strand 25 33 strand 43 51 helix 54 60 strand 78 90 strand 98 111 strand 118 120 helix 127 131 turn 132 134 helix 136 146 turn 147 149 strand 156 161 helix 167 177 strand 180 187 helix 188 196 strand 201 205 helix 206 208 helix 212 219 strand 224 231 helix 234 240 strand 243 251 helix 255 257 helix 271 274 strand 278 281 helix 284 290 helix 291 303 strand 311 316 helix 317 319 helix 320 326 helix 327 329 strand 333 339 Keyword 3D-structure,Direct protein sequencing,NADP,Oxidoreductase Sequence Source UniProt Protein Sequence MAEEVSNKQV ILKNYVTGYP KESDMEIKNV TIKLKVPEGS NDVVVKNLYL SCDPYMRSRM 60 RKIEGSYVES FAPGSPITGY GVAKVLESGD PKFQKGDLVW GMTGWEEYSI ITPTQTLFKI 120 HDKDVPLSYY TGILGMPGMT AYAGFHEVCS PKKGETVFVS AASGAVGQLV GQFAKMLGCY 180 VVGSAGSKEK VDLLKSKFGF DEAFNYKEEQ DLSAALKRYF PDGIDIYFEN VGGKMLDAVL 240 VNMKLYGRIA VCGMISQYNL EQTEGVHNLF CLITKRIRME GFLVFDYYHL YPKYLEMVIP 300 QIKAGKVVYV EDVAHGLESA PTALVGLFSG RNIGKQVVMV SRE 343 Gene Ontology GO:0016491; F: oxidoreductase activity; IEA: UniProtKB-KW GO:0008270; F: zinc ion binding; IEA: InterPro Interpro Pfam Pfam; PF00107; ADH_zinc_N SMART PROSITE PRINTS |