※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
6.55 
Molecular Weight
38088.1 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
2-alkenal reductase (NADP(+)-dependent); Alkenal double bound reductase; Allylic alcohol dehydrogenase 1; allyl-ADH1; Flavin-free double bond reductase; NtDBR; Pulegone reductase; NtRed-1 
Gene Name
DBR 
Created Date
2013-07-24 
Organism
Nicotiana tabacum(Common tobacco) 
NCBI Taxa ID
4097 
Phosphorylation Sites
Position
Peptide
Source
References
75
VESFAPGSPITGYGV
dbPPT, P3DB
[1, 2]
Functional Description
Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones. 
Sequence Annotation
chain 1 343 2-alkenal reductase (NADP(+)-dependent)
nucleotide phosphate-binding region 165 166 NADP
nucleotide phosphate-binding region 282 284 NADP
binding site 55 Substrate
binding site 80 Substrate
binding site 186 NADP; via amide nitrogen
binding site 190 NADP
binding site 206 NADP
binding site 230 NADP
binding site 252 NADP
binding site 258 NADP
binding site 332 NADP
strand 4 12
strand 17 19
helix 22 24
strand 25 33
strand 43 51
helix 54 60
strand 78 90
strand 98 111
strand 118 120
helix 127 131
turn 132 134
helix 136 146
turn 147 149
strand 156 161
helix 167 177
strand 180 187
helix 188 196
strand 201 205
helix 206 208
helix 212 219
strand 224 231
helix 234 240
strand 243 251
helix 255 257
helix 271 274
strand 278 281
helix 284 290
helix 291 303
strand 311 316
helix 317 319
helix 320 326
helix 327 329
strand 333 339
Keyword
3D-structure,Direct protein sequencing,NADP,Oxidoreductase 
Sequence Source
UniProt 
Protein Sequence
MAEEVSNKQV ILKNYVTGYP KESDMEIKNV TIKLKVPEGS NDVVVKNLYL SCDPYMRSRM 60
RKIEGSYVES FAPGSPITGY GVAKVLESGD PKFQKGDLVW GMTGWEEYSI ITPTQTLFKI 120
HDKDVPLSYY TGILGMPGMT AYAGFHEVCS PKKGETVFVS AASGAVGQLV GQFAKMLGCY 180
VVGSAGSKEK VDLLKSKFGF DEAFNYKEEQ DLSAALKRYF PDGIDIYFEN VGGKMLDAVL 240
VNMKLYGRIA VCGMISQYNL EQTEGVHNLF CLITKRIRME GFLVFDYYHL YPKYLEMVIP 300
QIKAGKVVYV EDVAHGLESA PTALVGLFSG RNIGKQVVMV SRE 343 
Gene Ontology
GO:0016491; F: oxidoreductase activity; IEA: UniProtKB-KW
GO:0008270; F: zinc ion binding; IEA: InterPro 
Interpro
InterPro; IPR013149; ADH_C
InterPro; IPR002085; ADH_SF_Zn-type
InterPro; IPR011032; GroES-like
InterPro; IPR016040; NAD(P)-bd_dom
Pfam
Pfam; PF00107; ADH_zinc_N 
SMART
 
PROSITE
 
PRINTS