dbPPT: Database of Phospho-sites in Plants

※ dbPPT Protein Information

Tag

Content

UniProt Accession

Q9T074; PCKA_ARATH

Theoretical PI

6.61

Molecular Weight

73405.09

Genbank Protein ID

AL161592;AY078035;AF372922;AL035709;CP002687

Genbank Nucleotide ID

CAB38935.1;AAK50062.1;CAB80452.1;AEE86847.1;AAL77736.1

Protein Name

Phosphoenolpyruvate carboxykinase [ATP]; PEP carboxykinase; PEPCK

Gene Name

PCKA; At4g37870; T28I19.150

Created Date

2001-01-11

Organism

Arabidopsis thaliana(Mouse-ear cress)

NCBI Taxa ID

3702

Phosphorylation Sites

Position	Peptide	Source	References
122	KKTDGSTTPAYAHGQ	dbPPT, P³DB, PhosPhAt	[15, 16, 8, 9]
138	HSIFSPATGAVSDSS	dbPPT, P³DB, PhosPhAt	[15]
2	******MSAGNGNAT	dbPPT, P³DB, PhosPhAt	[7]
135	GQHHSIFSPATGAVS	dbPPT, P³DB, PhosPhAt	[15]
62	HSLQKKRSAPTTPIN	dbPPT, P³DB, PhosPhAt	[1, 10, 11, 13, 15, 16, 2, 3, 4, 5, 7, 9]
407	SNIEGGCYAKCVDLS	dbPPT, P³DB, PhosPhAt	[14]
65	QKKRSAPTTPINQNA	dbPPT, P³DB, PhosPhAt	[1, 10, 11, 13, 15, 16, 3, 4, 7, 9]
80	AAAFAAVSEEERQKI	dbPPT, P³DB, PhosPhAt	[13, 4]
120	AEKKTDGSTTPAYAH	dbPPT, P³DB, PhosPhAt	[15, 16, 9]
132	YAHGQHHSIFSPATG	dbPPT, P³DB, PhosPhAt	[1, 12, 13, 15, 9]
117	GDPAEKKTDGSTTPA	dbPPT, P³DB, PhosPhAt	[15, 8]
125	DGSTTPAYAHGQHHS	dbPPT, P³DB, PhosPhAt	[15, 6, 8]
66	KKRSAPTTPINQNAA	dbPPT, P³DB, PhosPhAt	[1, 10, 11, 13, 15, 16, 2, 3, 4, 7, 9]
121	EKKTDGSTTPAYAHG	dbPPT, P³DB, PhosPhAt	[15, 7, 8]

Functional Description

Sequence Annotation

initiator methionine 1 Removed
chain 2 671 Phosphoenolpyruvate carboxykinase [ATP]
nucleotide phosphate-binding region 369 376 ATP
modified residue 2 N-acetylserine
modified residue 62 Phosphoserine
modified residue 66 Phosphothreonine

Keyword

Acetylation,ATP-binding,Complete proteome,Cytoplasm,Decarboxylase,Gluconeogenesis,Lyase,Nucleotide-binding,Phosphoprotein,Reference proteome

Sequence Source

UniProt

Protein Sequence

MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK 60
RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT RESGPKVVRG DPAEKKTDGS 120
TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA 180
TLSGAKTGRA PRDKRVVRDA TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF 240
VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN 300
RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK 360
DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS NIEGGCYAKC VDLSREKEPD 420
IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL 480
LACDAFGVLP PVSKLNLAQT MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT 540
KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT 600
EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL 660
TEEILAAGPI F 671

Gene Ontology

GO:0005829; C: cytosol; IDA: TAIR
GO:0016020; C: membrane; IDA: TAIR
GO:0005730; C: nucleolus; IDA: TAIR
GO:0005634; C: nucleus; IDA: TAIR
GO:0005524; F: ATP binding; IEA: UniProtKB-KW
GO:0004612; F: phosphoenolpyruvate carboxykinase (ATP) activity; IMP: TAIR
GO:0016036; P: cellular response to phosphate starvation; IEP: TAIR
GO:0009817; P: defense response to fungus, incompatible interaction; IDA: TAIR
GO:0006094; P: gluconeogenesis; IEA: UniProtKB-UniPathway
GO:0046686; P: response to cadmium ion; IEP: TAIR

Interpro

InterPro; IPR001272; PEP_carboxykinase_ATP
InterPro; IPR013035; PEP_carboxykinase_C
InterPro; IPR008210; PEP_carboxykinase_N
InterPro; IPR015994; PEPCK_ATP_CS

Pfam

Pfam; PF01293; PEPCK_ATP

SMART

PROSITE

PS00532; PEPCK_ATP

PRINTS