UniProt Accession

Q9XIE6; Q8RWQ3; ALA3_ARATH 

Theoretical PI

7.84 

Molecular Weight

137752.78 

Genbank Protein ID

AY091777;CP002684;AC007258 

Genbank Nucleotide ID

AEE33621.1;AAM10325.1;AAD39325.1 

Protein Name

Phospholipid-transporting ATPase 3; AtALA3; Aminophospholipid ATPase 3; Aminophospholipid flippase 3; Protein IRREGULAR TRICHOME BRANCH 2 

Gene Name

ALA3; ITB2; At1g59820; F23H11.14 

Created Date

2001-01-11 

Organism

Arabidopsis thaliana(Mouse-ear cress) 

NCBI Taxa ID

3702 

Phosphorylation Sites

Position	Peptide	Source	References
22	HQRTPSRTVTLGHIQ	dbPPT, P3DB, PhosPhAt	[3]
4	****MVRSGSFSVDS	dbPPT, P3DB, PhosPhAt	[3]
962	FEKDVSASLSKRYPE	dbPPT, P3DB, PhosPhAt	[1, 2, 4]
24	RTPSRTVTLGHIQPQ	dbPPT, P3DB, PhosPhAt	[2, 3, 4, 5]

Functional Description

Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid. 

Sequence Annotation

chain 1 1213 Phospholipid-transporting ATPase 3
topological domain 1 67 Cytoplasmic
transmembrane region 68 89 Helical;
topological domain 90 93 Extracellular
transmembrane region 94 116 Helical;
topological domain 117 297 Cytoplasmic
transmembrane region 298 319 Helical;
topological domain 320 346 Extracellular
transmembrane region 347 364 Helical;
topological domain 365 900 Cytoplasmic
transmembrane region 901 920 Helical;
topological domain 921 934 Extracellular
transmembrane region 935 954 Helical;
topological domain 955 984 Cytoplasmic
transmembrane region 985 1006 Helical;
topological domain 1007 1013 Extracellular
transmembrane region 1014 1036 Helical;
topological domain 1037 1042 Cytoplasmic
transmembrane region 1043 1063 Helical;
topological domain 1064 1076 Extracellular
transmembrane region 1077 1087 Helical;
topological domain 1088 1213 Cytoplasmic
active site 413 4-aspartylphosphate intermediate
metal ion-binding site 845 Magnesium
metal ion-binding site 849 Magnesium

Keyword

ATP-binding,Complete proteome,Endoplasmic reticulum,Golgi apparatus,Hydrolase,Magnesium,Membrane,Metal-binding,Nucleotide-binding,Reference proteome,Transmembrane,Transmembrane helix 

Sequence Source

UniProt 

Protein Sequence

Gene Ontology

GO:0005789; C: endoplasmic reticulum membrane; IEA: UniProtKB-SubCell
GO:0005768; C: endosome; IDA: TAIR
GO:0005794; C: Golgi apparatus; IDA: TAIR
GO:0000139; C: Golgi membrane; IEA: UniProtKB-SubCell
GO:0016021; C: integral component of membrane; IEA: UniProtKB-KW
GO:0005886; C: plasma membrane; IDA: TAIR
GO:0005802; C: trans-Golgi network; IDA: TAIR
GO:0005524; F: ATP binding; IEA: UniProtKB-KW
GO:0019829; F: cation-transporting ATPase activity; IEA: InterPro
GO:0000287; F: magnesium ion binding; IEA: InterPro
GO:0005548; F: phospholipid transporter activity; IDA: TAIR
GO:0004012; F: phospholipid-translocating ATPase activity; IEA: UniProtKB-EC
GO:0048194; P: Golgi vesicle budding; IMP: TAIR
GO:0048364; P: root development; IMP: TAIR
GO:0048367; P: shoot system development; IMP: TAIR 

Interpro

InterPro; IPR023299; ATPase_P-typ_cyto_domN
InterPro; IPR018303; ATPase_P-typ_P_site
InterPro; IPR006539; ATPase_P-typ_Plipid-transp
InterPro; IPR008250; ATPase_P-typ_transduc_dom_A
InterPro; IPR001757; Cation_transp_P_typ_ATPase
InterPro; IPR023214; HAD-like_dom

Pfam

Pfam; PF00122; E1-E2_ATPase 

SMART

 

PROSITE

PS00154; ATPASE_E1_E2 

PRINTS

PR00119; CATATPASE