※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||
Theoretical PI | 9.24 | ||||||||||||||||||||||||||||||||||
Molecular Weight | 78840.06 | ||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||
Protein Name | Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2; AtMPF2; Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase | ||||||||||||||||||||||||||||||||||
Gene Name | MFP2; At3g06860; F17A9.1 | ||||||||||||||||||||||||||||||||||
Created Date | 2010-11-30 | ||||||||||||||||||||||||||||||||||
Organism | Arabidopsis thaliana(Mouse-ear cress) | ||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 3702 | ||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Involved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18). Sequence Annotation chain 1 725 Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2 short sequence motif 723 725 Microbody targeting signal active site 119 Nucleophile active site 139 Proton acceptor strand 8 13 strand 17 24 turn 26 29 helix 33 46 strand 54 62 strand 85 87 helix 88 92 helix 93 99 strand 101 103 strand 105 109 strand 111 114 helix 116 123 strand 124 129 strand 134 136 helix 139 142 helix 150 158 helix 160 169 helix 175 180 strand 185 187 turn 190 192 helix 193 205 helix 214 216 helix 224 241 helix 247 260 helix 263 277 helix 281 294 helix 295 297 turn 300 302 strand 303 305 strand 315 318 helix 322 332 turn 333 335 strand 338 341 helix 345 361 helix 372 375 turn 376 378 strand 379 386 helix 387 389 strand 393 397 helix 403 416 strand 422 425 strand 428 430 helix 432 435 turn 436 438 turn 442 444 strand 445 450 turn 454 456 strand 459 464 helix 470 482 strand 486 493 turn 494 497 helix 498 514 helix 519 529 helix 535 542 helix 544 557 helix 559 561 helix 567 572 helix 605 610 turn 618 621 helix 624 644 strand 647 649 helix 651 662 helix 666 668 helix 671 678 helix 680 694 helix 696 698 helix 702 710 strand 714 716 Keyword 3D-structure,Complete proteome,Fatty acid metabolism,Glyoxysome,Isomerase,Lipid metabolism,Lyase,Multifunctional enzyme,NAD,Oxidoreductase,Peroxisome,Reference proteome Sequence Source UniProt Protein Sequence MDSRTKGKTV MEVGGDGVAV ITLINPPVNS LSFDVLYNLK SNYEEALSRN DVKAIVITGA 60 KGRFSGGFDI SGFGEMQKGN VKEPKAGYIS IDIITDLLEA ARKPSVAAID GLALGGGLEL 120 AMACHARISA PAAQLGLPEL QLGVIPGFGG TQRLPRLVGL TKALEMILTS KPVKAEEGHS 180 LGLIDAVVPP AELVTTARRW ALDIVGRRKP WVSSVSKTDK LPPLGEAREI LTFAKAQTLK 240 RAPNMKHPLM CLDAIEVGIV SGPRAGLEKE AEVASQVVKL DTTKGLIHVF FSQRGTAKVP 300 GVTDRGLVPR KIKKVAIIGG GLMGSGIATA LILSNYPVIL KEVNEKFLEA GIGRVKANLQ 360 SRVRKGSMSQ EKFEKTMSLL KGSLDYESFR DVDMVIEAVI ENISLKQQIF ADLEKYCPQH 420 CILASNTSTI DLNKIGERTK SQDRIVGAHF FSPAHIMPLL EIVRTNHTSA QVIVDLLDVG 480 KKIKKTPVVV GNCTGFAVNR MFFPYTQAAM FLVECGADPY LIDRAISKFG MPMGPFRLCD 540 LVGFGVAIAT ATQFIENFSE RTYKSMIIPL MQEDKRAGEA TRKGFYLYDD KRKAKPDPEL 600 KKYIEKARSI SGVKLDPKLA NLSEKDIIEM TFFPVVNEAC RVFAEGIAVK AADLDIAGIM 660 GMGFPPYRGG IMFWADSIGS KYIYSRLDEW SKAYGEFFKP CAFLAERGSK GVLLSAPVKQ 720 ASSRL 725 Gene Ontology GO:0005618; C: cell wall; IDA: TAIR GO:0009514; C: glyoxysome; IEA: UniProtKB-SubCell GO:0005730; C: nucleolus; IDA: TAIR GO:0005777; C: peroxisome; IDA: TAIR GO:0009506; C: plasmodesma; IDA: TAIR GO:0003857; F: 3-hydroxyacyl-CoA dehydrogenase activity; IDA: UniProtKB GO:0008692; F: 3-hydroxybutyryl-CoA epimerase activity; IEA: UniProtKB-EC GO:0050662; F: coenzyme binding; IEA: InterPro GO:0004165; F: dodecenoyl-CoA delta-isomerase activity; IEA: UniProtKB-EC GO:0004300; F: enoyl-CoA hydratase activity; IEA: UniProtKB-EC GO:0016508; F: long-chain-enoyl-CoA hydratase activity; IDA: UniProtKB GO:0006635; P: fatty acid beta-oxidation; IEP: TAIR Interpro InterPro; IPR006180; 3-OHacyl-CoA_DH_CS InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd InterPro; IPR006108; 3HC_DH_C InterPro; IPR008927; 6-PGluconate_DH_C-like InterPro; IPR029045; ClpP/crotonase-like_dom InterPro; IPR001753; Crotonase_core_superfam InterPro; IPR013328; DH_multihelical InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS InterPro; IPR016040; NAD(P)-bd_dom Pfam SMART PROSITE PRINTS |