※ dbPPT Protein Information

TagContent
UniProt Accession
Theoretical PI
9.36 
Molecular Weight
77858.08 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1; Protein ABNORMAL INFLORESCENCE MERISTEM 1; AtAIM1; Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
AIM1; At4g29010; F19B15.40 
Created Date
2010-11-30 
Organism
Arabidopsis thaliana(Mouse-ear cress) 
NCBI Taxa ID
3702 
Phosphorylation Sites
Position
Peptide
Source
References
326
LMGSGIATALLLSNI
dbPPT, PhosPhAt
[1, 2]
331
IATALLLSNIRVVLK
dbPPT, PhosPhAt
[1, 2]
712
AMNGMLLSESKSSRS
dbPPT, PhosPhAt
[2]
Functional Description
Involved in peroxisomal fatty acid beta-oxidation. Required for wound-induced jasmonate biosynthesis. Possesses enoyl-CoA hydratase activity against short chain substrates (C4-C6) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C16). 
Sequence Annotation
chain 1 721 Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1
active site 116 Nucleophile
active site 136 Proton acceptor
Keyword
Complete proteome,Fatty acid metabolism,Isomerase,Lipid metabolism,Lyase,Multifunctional enzyme,NAD,Oxidoreductase,Peroxisome,Reference proteome 
Sequence Source
UniProt 
Protein Sequence
MAKKIGVTME VGNDGVAVIT ISNPPVNSLA SPIISGLKEK FRDANQRNDV KAIVLIGNNG 60
RFSGGFDINV FQQVHKTGDL SLMPEVSVEL VCNLMEDSRK PVVAAVEGLA LGGGLELAMA 120
CHARVAAPKA QLGLPELTLG VIPGFGGTQR LPRLVGLAKA TDMILLSKSI SSEEGHKLGL 180
IDALVPPGDV LSTSRKWALD IAEGRKPFLQ SLHRTDKIGS LSEARAILKN SRQLAKKIAP 240
NMPQHHACIE VIEEGIIHGG YSGVLKEAEV FKQLVLSDTA KGLVHVFFAQ RATSKVPNVT 300
DVGLKPRPIK KVAVIGGGLM GSGIATALLL SNIRVVLKEI NSEFLMKGIK SVEANMKSLV 360
SRGKLTQDKA GKALSLFKGV LDYTEFNDVD MVIEAVIENI QLKQNIFKEI EKVCSPHCIL 420
ASNTSTIDLD VIGEKTNSKD RIVGAHFFSP AHLMPLLEIV RSKNTSAQVI LDLMAVGKAI 480
KKVPVVVGNC IGFAVNRTFF PYSQAAHMLA NLGVDLFRID SVITSFGLPL GPFQLGDLAG 540
HGIGLAVGPI YAKVYGDRMF RSPMTELLLK SGRNGKINGR GYYIYEKGSK PKPDPSVLSI 600
VEKSRKLTNI MPGGKPISVT DKEIVEMILF PVVNEACRVL DEGVVIRASD LDIASVLGMS 660
FPSYRGGIVF WADTVGPKYI YERLKKLSET YGSFFKPSRY LEERAMNGML LSESKSSRSK 720
L 721 
Gene Ontology
GO:0005618; C: cell wall; IDA: TAIR
GO:0009507; C: chloroplast; IDA: TAIR
GO:0005777; C: peroxisome; IDA: TAIR
GO:0009506; C: plasmodesma; IDA: TAIR
GO:0003857; F: 3-hydroxyacyl-CoA dehydrogenase activity; IDA: UniProtKB
GO:0008692; F: 3-hydroxybutyryl-CoA epimerase activity; IEA: UniProtKB-EC
GO:0050662; F: coenzyme binding; IEA: InterPro
GO:0004165; F: dodecenoyl-CoA delta-isomerase activity; IEA: UniProtKB-EC
GO:0004300; F: enoyl-CoA hydratase activity; IDA: UniProtKB
GO:0006635; P: fatty acid beta-oxidation; ISS: TAIR
GO:0009908; P: flower development; IMP: TAIR
GO:0009695; P: jasmonic acid biosynthetic process; IMP: UniProtKB 
Interpro
InterPro; IPR006180; 3-OHacyl-CoA_DH_CS
InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd
InterPro; IPR006108; 3HC_DH_C
InterPro; IPR008927; 6-PGluconate_DH_C-like
InterPro; IPR029045; ClpP/crotonase-like_dom
InterPro; IPR001753; Crotonase_core_superfam
InterPro; IPR013328; DH_multihelical
InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS
InterPro; IPR016040; NAD(P)-bd_dom
Pfam
Pfam; PF00725; 3HCDH
Pfam; PF02737; 3HCDH_N
Pfam; PF00378; ECH 
SMART
 
PROSITE
PS00067; 3HCDH
PS00166; ENOYL_COA_HYDRATASE 
PRINTS