※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||||||
Theoretical PI | 6.21 | ||||||||||||||||||||||||||||||||||||||
Molecular Weight | 48713.69 | ||||||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||||||
Protein Name | Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; Glutamate N-acetyltransferase; GAT; Ornithine acetyltransferase; OATase; Ornithine transacetylase; Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; Arginine biosynthesis bifunctional protein ArgJ alpha chain; Arginine biosynthesis bifunctional protein ArgJ beta chain | ||||||||||||||||||||||||||||||||||||||
Gene Name | At2g37500; F3G5.29 | ||||||||||||||||||||||||||||||||||||||
Created Date | 2010-10-05 | ||||||||||||||||||||||||||||||||||||||
Organism | Arabidopsis thaliana(Mouse-ear cress) | ||||||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 3702 | ||||||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate (By similarity). Sequence Annotation transit peptide 1 36 Chloroplast chain 37 248 Arginine biosynthesis bifunctional protein ArgJ alpha chain chain 249 468 Arginine biosynthesis bifunctional protein ArgJ beta chain active site 249 Nucleophile binding site 212 Substrate binding site 238 Substrate binding site 249 Substrate binding site 336 Substrate binding site 463 Substrate binding site 468 Substrate site 173 Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole site 174 Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole site 248 249 Cleavage; by autolysis splice variant 1 59 In isoform 2. splice variant 60 62 In isoform 2. Keyword Acyltransferase,Alternative splicing,Amino-acid biosynthesis,Arginine biosynthesis,Autocatalytic cleavage,Chloroplast,Complete proteome,Multifunctional enzyme,Plastid,Reference proteome,Transferase,Transit peptide Sequence Source UniProt Protein Sequence MHSCSHTHFV SFKLPHFFAP KSFVVSSRRE LRVFAVATTV EEASGNIPAA PISLPQGSWK 60 QIAGGVTAAK GFKAAGMYAG LRAAGKKPDL ALVTCDVEAV AAGVFTTNVV AAAPVVYCKK 120 VLETSKTARA VLINAGQANA ATGDAGYQDM LDCVGSIATL LKVKPEEVLI ESTGVIGQRI 180 KKEELLHALP TLVNSRSDSV EEADSAAVAI TTTDLVSKSV AVESQVGGIK IRVGGMAKGS 240 GMIHPNMATM LGVITTDALV ESDIWRKMVK VAVNRSFNQI TVDGDTSTND TVIALASGLS 300 GSPSISSLNC KEAAQLQACL DAVMQGLAKS IAWDGEGATC LIEVTVKGTE TEAEAAKIAR 360 SVASSSLVKA AVYGRDPNWG RIAAAAGYAG VSFQMDKLKI SLGEFSLMES GQPLPFDRDG 420 ASNYLKKTGE VHGTVTIDIS VGDGAAIGKA WGCDLSYDYV KINAEYTS 468 Gene Ontology GO:0009507; C: chloroplast; IDA: TAIR GO:0009570; C: chloroplast stroma; IDA: TAIR GO:0004042; F: acetyl-CoA: L-glutamate N-acetyltransferase activity; IEA: UniProtKB-HAMAP GO:0004358; F: glutamate N-acetyltransferase activity; IEA: UniProtKB-HAMAP GO:0006526; P: arginine biosynthetic process; IEA: UniProtKB-HAMAP Interpro Pfam Pfam; PF01960; ArgJ SMART PROSITE PRINTS |