※ dbPPT Protein Information
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UniProt Accession | |||||||||||||||||||||||||||||||||||||||||||||||||||
Theoretical PI | 9.05 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular Weight | 102840.44 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Genbank Protein ID | |||||||||||||||||||||||||||||||||||||||||||||||||||
Genbank Nucleotide ID | |||||||||||||||||||||||||||||||||||||||||||||||||||
Protein Name | Protein argonaute 4; Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Gene Name | AGO4; OCP11; At2g27040; T20P8.9 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Created Date | 2011-02-08 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Organism | Arabidopsis thaliana(Mouse-ear cress) | ||||||||||||||||||||||||||||||||||||||||||||||||||
NCBI Taxa ID | 3702 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Phosphorylation Sites |
Functional Description Involved in transcriptional gene silencing (TGS). Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses. Sequence Annotation chain 1 924 Protein argonaute 4 domain 272 408 PAZ domain 577 885 Piwi Keyword Complete proteome,Host-virus interaction,Nucleus,Plant defense,Reference proteome,Repressor,Ribonucleoprotein,RNA-binding,RNA-mediated gene silencing,Transcription,Transcription regulation,Translation regulation Sequence Source UniProt Protein Sequence MDSTNGNGAD LESANGANGS GVTEALPPPP PVIPPNVEPV RVKTELAEKK GPVRVPMARK 60 GFGTRGQKIP LLTNHFKVDV ANLQGHFFHY SVALFYDDGR PVEQKGVGRK ILDKVHQTYH 120 SDLDGKEFAY DGEKTLFTYG ALPSNKMDFS VVLEEVSATR ANGNGSPNGN ESPSDGDRKR 180 LRRPNRSKNF RVEISYAAKI PLQALANAMR GQESENSQEA IRVLDIILRQ HAARQGCLLV 240 RQSFFHNDPT NCEPVGGNIL GCRGFHSSFR TTQGGMSLNM DVTTTMIIKP GPVVDFLIAN 300 QNARDPYSID WSKAKRTLKN LRVKVSPSGQ EFKITGLSDK PCREQTFELK KRNPNENGEF 360 ETTEVTVADY FRDTRHIDLQ YSADLPCINV GKPKRPTYIP LELCALVPLQ RYTKALTTFQ 420 RSALVEKSRQ KPQERMTVLS KALKVSNYDA EPLLRSCGIS ISSNFTQVEG RVLPAPKLKM 480 GCGSETFPRN GRWNFNNKEF VEPTKIQRWV VVNFSARCNV RQVVDDLIKI GGSKGIEIAS 540 PFQVFEEGNQ FRRAPPMIRV ENMFKDIQSK LPGVPQFILC VLPDKKNSDL YGPWKKKNLT 600 EFGIVTQCMA PTRQPNDQYL TNLLLKINAK LGGLNSMLSV ERTPAFTVIS KVPTIILGMD 660 VSHGSPGQSD VPSIAAVVSS REWPLISKYR ASVRTQPSKA EMIESLVKKN GTEDDGIIKE 720 LLVDFYTSSN KRKPEHIIIF RDGVSESQFN QVLNIELDQI IEACKLLDAN WNPKFLLLVA 780 QKNHHTKFFQ PTSPENVPPG TIIDNKICHP KNNDFYLCAH AGMIGTTRPT HYHVLYDEIG 840 FSADELQELV HSLSYVYQRS TSAISVVAPI CYAHLAAAQL GTFMKFEDQS ETSSSHGGIT 900 APGPISVAQL PRLKDNVANS MFFC 924 Gene Ontology GO:0015030; C: Cajal body; IDA: TAIR GO:0005719; C: nuclear euchromatin; IDA: TAIR GO:0005730; C: nucleolus; IDA: TAIR GO:0030529; C: ribonucleoprotein complex; IEA: UniProtKB-KW GO:0005515; F: protein binding; IPI: UniProtKB GO:0035197; F: siRNA binding; IDA: TAIR GO:0006342; P: chromatin silencing; IMP: TAIR GO:0031048; P: chromatin silencing by small RNA; TAS: TAIR GO:0009816; P: defense response to bacterium, incompatible interaction; IMP: TAIR GO:0006306; P: DNA methylation; IMP: TAIR GO:0051567; P: histone H3-K9 methylation; IMP: TAIR GO:0030422; P: production of siRNA involved in RNA interference; IGI: TAIR GO:0006417; P: regulation of translation; IEA: UniProtKB-KW GO:0006351; P: transcription, DNA-templated; IEA: UniProtKB-KW GO:0016032; P: viral process; IEA: UniProtKB-KW Interpro Pfam SMART SMART; SM00950; Piwi PROSITE PRINTS |